siRNA Transfection Reagent from Santa Cruz Biotechnology, Inc.

Product Specs
ItemsiRNA Transfection Reagent
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-29528
Quantity0.3 ml
TypeTransfection Reagents and Kits

Specifications/Features

Advantages:
Enables highly efficient siRNA transfection

Effective in both serum-containing medium and serum-free medium

# of Transfections:
50-100 transfections (using 12-well plates)

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) USP13 drives lung squamous cell carcinoma by switching lung club cell lineage plasticityMol CancerDecember 13, 2023Juntae Kwon, Jinmin Zhang, Boram Mok, Samuel Allsup, Chul Kim, Jeffrey Toretsky, Cecil Han(Cat#sc-29226) were purchased from Santa Cruz and transfected into cells using siRNA Transfection Reagent (Cat#sc-29528) followed by the manufacturer’s instructions. Mouse tumor bulk RNA-seq Anesthetized KP and KPU mice

(2) Tools and tactics to define specificity of metabolic chemical reportersFront Mol BiosciDecember 7, 2023Mana Mohan Mukherjee, Michelle R. Bond, Lara K. Abramowitz, Devin Biesbrock, Carolyn C. Woodroofe, Eun Ju Kim, Rolf E. Swenson, John A. Hanoverwithout antibiotics. The following siRNA reagents were purchased from Santa Cruz: transfection reagent (sc-29528), NCOAT siRNA construct (human) (sc-62667), OGT siRNA construct (human) (sc-40780), and Control siRNA-A

(3) Host-Cell-Dependent Roles of E-Cadherin in Serratia InvasionInt J Mol SciDecember 3, 2023Olga Tsaplina, Ekaterina Lomert, Yuliya Berson(Santa Cruz, Dallas, TX, USA). Transfection of siRNAs was performed using siRNA Transfection Reagent (sc-29528) as recommended by the manufacturer (Santa Cruz, Dallas, TX, USA). The RNA interference efficiency was

(4) Expression of Claudin-9 (CLDN9) in Breast Cancer, the Clinical Significance in Connection with Its Subcoat Anchorage Proteins ZO-1 and ZO-3 and Impact on Drug ResistanceBiomedicinesNovember 24, 2023Xinguo Zhuang, Tracey A. Martin, Fiona Ruge, Jianyuan (Jimmy) Zeng, Xinyu (Amber) Li, Elyas Khan, Qingping Dou, Eleri Davies, Wen G. JiangUUUCCAGUUACAAAUGCUCtt. Transfection of cancer cells was carried out using transfection kits (SC-36868 and SC-29528) purchased from Santa Cruz Biotechnology Inc., by following the manufacturer’s instructions. The effect

(5) Inhibition of HDAC6 alleviates cancer‑induced bone pain by reducing the activation of NLRP3 inflammasomeInternational Journal of Molecular MedicineNovember 17, 2023Yin-Di Hu, Zhao-Di Wang, Yuan-Fen Yue, Dai Li, Shu-Qing Zhen, Jie-Qiong Ding, Wei Meng, Hai-Li Zhu, Min Xie, Ling Liuinterfering (si) RNA (sc-35544), siRNA Transfection Medium (sc-36868), siRNA Transfection Reagent (sc-29528) and control siRNA (sc-37007) were both purchased from Santa Cruz Biotechnology, Inc. Animals A total

(6) The inhibitory effect of trastuzumab on BT474 triple‑positive breast cancer cell viability is reversed by the combination of progesterone and estradiolOncology LettersNovember 15, 2023José A. López‑Méndez, José L. Ventura‑Gallegos, Ignacio Camacho‑Arroyo, Marcela Lizano, Alberto J. Cabrera‑Quintero, Sandra L. Romero‑Córdoba, Mariano Martínez‑Vázquez, Nadia J. Jacobo‑Herrera, Alfonso León‑Del‑Río, Adrian A. Paredes‑Villa, et al.Santa Cruz Biotechnology, Inc.) using 6 µl of a commercially available siRNA transfection reagent (cat. no. sc-29528; Santa Cruz Biotechnology, Inc.). Immediately after the transfection period, the subsequent experiments

(7) Fyn-Mediated Paxillin Tyrosine 31 Phosphorylation Regulates Migration and Invasion of Breast Cancer CellsInternational Journal of Molecular SciencesNovember 5, 2023Ying Zhang, Huanyu Zheng, Ming Xu, Noriko Maeda, Ryouichi Tsunedomi, Hiroko Kishi, Hiroaki Nagano, Sei Kobayashiwas obtained from Qiagen. Cells were transfected with 40 nM siRNA using a siRNA transfection reagent (sc-29528, Santa Cruz, Dallas, CA, USA) according to the manufacturer’s instructions. After the siRNA transfection,

(8) Cav3.2 T-Type calcium channels downregulation attenuates bone cancer pain induced by inhibiting IGF-1/HIF-1α signaling pathway in the rat spinal cordJournal of Bone OncologyOctober 1, 2023Qingying Liu, Zhongyuan Lu, Huan Ren, Lijun Fu, Yueliang Wang, Huilian Bu, Minyu Ma, Letian Ma, Chen Huang, Jian Wang, et al.dissolved in RNase-free water at 1 μg/μl as a stock solution and mixed with the transfection reagent (sc29528, Santa Cruz Biotechnology) before injection, following the manufacturer’s instruction. JB-1 (147819-32-7,

(9) Methylmercury (MeHg) transcriptionally regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) in Hepa-1c1c7 cellsCurrent Research in ToxicologySeptember 17, 2023Mohammed A. Alqahtani, Mahmoud A. El-Ghiaty, Sara R. El-Mahrouk, Ayman O.S. El-Kadi(sc-377000), Nrf2 siRNA (sc-37049), siRNA transfection medium (sc-36868), siRNA transfection reagent (sc-29528) control siRNA-A (sc-37007) are from Santa Cruz Biotechnology (Santa Cruz, CA). All remaining materials

(10) Myo1d promotes alpha-synuclein transfer from brain microvascular endothelial cells to pericytes through tunneling nanotubesiScienceAugust 18, 2023Duan Q, Zhang Q, Nie K, Huang R, Yang J, He P, Tie Z, Huang H, Ma G, Zhang Y, et al.(Santa Cruz Biotechnology, sc-36868), and then mixed with 6 μl of siRNA Transfection Reagent (Santa Cruz Biotechnology, sc-29528) diluted in siRNA Transfection Medium. The cells were washed once with 2 ml of siRNA Transfection Medium,

(11) Adenosine Methylation Level of miR-125a-5p Promotes Anti-PD-1 Therapy Escape through the Regulation of IGSF11/VSIG3 ExpressionCancers (Basel)June 14, 2023Gwenola Bougras-Cartron, Arulraj Nadaradjane, Marie-Pierre Joalland, Lisenn Lalier-Bretaudeau, Judith Raimbourg, Pierre-François Cartron37 °C in a CO2 incubator. Then, 60 pmol of siRNA mixture (prepared with siRNA Transfection reagent, #sc-29528, Santa Cruz, France) was incubated on cells for 7 h at 37 °C in a CO2 incubator. Without removing the

(12) Involvement of Lipid Rafts in the Invasion of Opportunistic Bacteria Serratia into Eukaryotic CellsInternational Journal of Molecular SciencesMay 20, 2023Yuliya Berson, Sofia Khaitlina, Olga Tsaplina(Santa Cruz, Heidelberg, Germany). Transfection of siRNAs was performed using a siRNA Transfection Reagent (sc-29528) as recommended by the manufacturer (Santa Cruz, Heidelberg, Germany). The RNA interference efficiency

(13) Oscillating Glucose Induces the Increase in Inflammatory Stress through Ninjurin-1 Up-Regulation and Stimulation of Transport Proteins in Human Endothelial CellsBiomoleculesMarch 30, 2023Laura Toma, Gabriela M. Sanda, Camelia S. Stancu, Loredan S. Niculescu, Mina Raileanu, Anca V. SimaNINJ-1 siRNA (siNinj, sc-75915), control scramble siRNA (Scr, sc-37007) and siRNA Transfection Reagent (sc-29528) were from Santa Cruz Biotechnology, Santa Cruz, CA, USA, and Oligofectamine™ Transfection Reagent (12252011)

(14) ADAR1 has an oncogenic function and can be a prognostic factor in cervical cancerScientific ReportsMarch 23, 2023Keiichiro Nakamura, Kunitoshi Shigeyasu, Kazuhiro Okamoto, Hirofumi Matsuoka, Hisashi Masuyamavector (mock) for gene silencing (final concentration, 100 nmol/L) using an siRNA transfection reagent (sc-29528; Santa Cruz Biotechnology, TX, USA). RNA isolation and real-time quantitative PCR analyses Total RNA

(15) p53 Regulates Mitochondrial Dynamics in Vascular Smooth Muscle Cell CalcificationInternational journal of molecular sciencesJanuary 13, 2023Phadwal K, Tang QY, Luijten I, Zhao JF, Corcoran B, Semple RK, Ganley IG, MacRae VE.USA) or scrambled control siRNA (SC-37007; Santa Cruz Biotechnology) with siRNA transfection reagent (SC-29528; Santa Cruz Biotechnology) following the manufacturer’s protocol. For p53 overexpression, Trp53_OMu22609D_pcDNA3.1+/C-(K)-DYK,

(16) The short-chain fatty acid butyrate exerts a specific effect on VE-cadherin phosphorylation and alters the integrity of aortic endothelial cellsFrontiers in cell and developmental biologyJanuary 1, 2023Guo J, Terhorst I, Stammer P, Ibrahim A, Oberhuber A, Eierhoff T.sc-37007), siRNA transfection medium (Santa Cruz, sc-36868), siRNA transfection reagent (Santa Cruz, sc-29528), siRNA dilution buffer (sc-29517), VE-cadherin siRNA (h) (Santa Cruz, sc-36814), GPR41 siRNA(h) (Santa Cruz

(17) Angiotensin II type 2 receptor prevents extracellular matrix accumulation in human peritoneal mesothelial cell by ameliorating lipid disorder via LOXâ€1 suppressionRenal FailureDecember 31, 2022Jing Liu, Bo Jin, Jian Lu, Yuan Feng, Nan Li, Cheng Wan, Qing-Yan Zhang, Chun-Ming JiangsiRNA (sc-29752), LOX-1 siRNA (sc-40185), negative control siRNA (sc-36869) and normal control siRNA (sc-29528) following the manufacturer’s protocol from Santa Cruz Biotechnology. Before seeding in a well containing

(18) Construction and validation of a novel algorithm based on oncosis‑related lncRNAs comprising the immune landscape and prediction of colorectal cancer prognosisOncology LettersDecember 23, 2022Haoyi Xiang, Xuning Shen, Engeng Chen, Wei Chen, Zhangfa Songreached 80% confluence, and they were then transfected with siRNA (siRNA Transfection Reagent; cat. no. sc-29528; Santa Cruz Biotechnology, Inc.) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.).

(19) Transcriptomic analysis of a 3D blood–brain barrier model exposed to disturbed fluid flowFluids and Barriers of the CNSNovember 24, 2022Nesrine Bouhrira, Brandon J. DeOre, Kiet A. Tran, Peter A. Galie200 k per well until they reached 60–80% confluency. A mixture of transfection reagent (Santa Cruz, sc-29528) and transfection media (Santa Cruz, sc-36868) was added to the transfection solution, and then to the

(20) A novel monocyte differentiation pattern in pristane-induced lupus with diffuse alveolar hemorrhageeLifeOctober 20, 2022Shuhong Han, Haoyang Zhuang, Rawad Daniel Arja, Westley H Reevessterile water) was added to 100 µl of Opti-MEM medium and transfected using siRNA transfection reagent (sc-29528, Santa Cruz Biotechnology) following the manufacturer’s instructions. Transfected cells were incubated

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siRNA Transfection Reagent from Santa Cruz Biotechnology, Inc.

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Specifications/Features

Santa Cruz Biotechnology, Inc.

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siRNA Transfection Reagent from Santa Cruz Biotechnology, Inc.

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